Senescence‐inducible LEC2 enhances triacylglycerol accumulation in leaves without negatively affecting plant growth

• DOI: 10.1111/pbi.12354
• Publication Date: 2015-03-18T15:07:01Z
• Authors: Hyun Uk Kim; Kyeong‐Ryeol Lee; Su‐Jin Jung; Hyun A Shin; Young Sam Go; Mi‐Chung Suh; Jong Bum Kim
• Keywords: Senescence; Wild type; Ectopic expression

Summary The synthesis of fatty acids and glycerolipids in wild‐type Arabidopsis leaves does not typically lead to strong triacylglycerol ( TAG ) accumulation. LEAFY COTYLEDON 2 LEC 2) is a master regulator seed maturation oil accumulation seeds. Constitutive ectopic expression causes somatic embryogenesis defects seedling growth. Here, we report that senescence‐inducible caused threefold increase levels transgenic compared with the plants. Plant growth was severely affected by response expression. plastid‐synthesized lipids, mono‐ di‐galactosyldiacylglycerol phosphatidylglycerol were reduced more senescence‐induced than endoplasmic reticulum‐synthesized including phosphatidylcholine, phosphatidylethanolamine phosphatidylinositol. Senescence‐induced up‐regulated many genes involved acid biosynthesis at precise times senescent leaves, WRINKLED 1 WRI ), which encodes transcription factor. expressions glycerol‐3‐phosphate dehydrogenase phospholipid:diacylglycerol increased leaves. Five seed‐type oleosin‐encoding genes, expressed during oil‐body formation, seed‐specific FAE gene, enzyme responsible for C20:1 C22:1 acids, also higher senescing Senescence‐inducible triggers key metabolic steps vegetative tissues.