PbrmiR397a regulates lignification during stone cell development in pear fruit

• DOI: 10.1111/pbi.12950
• Publication Date: 2018-05-13T07:21:10Z
• Authors: Cheng Xue; Jia‐Long Yao; Meng‐Fan Qin; Ming‐Yue Zhang; Andrew C. Allan; De‐Fu Wang; Jun Wu
• Keywords: Nicotiana; 2. Zero hunger; 0303 health sciences; Sequence Analysis, DNA; Genes, Plant; Plants, Genetically Modified; Lignin; Pyrus; MicroRNAs; 03 medical and health sciences; Gene Expression Regulation, Plant; Fruit; Research Articles; Phylogeny

Lignified stone cells substantially reduce fruit quality. Therefore, it is desirable to inhibit cell development using genetic technologies. However, the molecular mechanisms regulating lignification are poorly understood in cells. In this study, we have shown that microRNA (miR) miR397a regulates by inhibiting laccase (LAC) genes encode key lignin biosynthesis enzymes. Transient overexpression of PbrmiR397a, which Chinese pear (Pyrus bretschneideri), and simultaneous silencing three LAC reduced content number fruit. A single nucleotide polymorphism (SNP) identified promoter PbrmiR397a gene was found associate with low levels lignin, after analysis genome sequences sixty varieties. This SNP created a TCA element responded salicylic acid induce expression as confirmed cell-based assay system. Furthermore, stable transgenic tobacco plants target decreased but did not change ratio syringyl- guaiacyl-lignin monomers. Consistent reduction content, showed fewer numbers vessel elements thinner secondary walls remaining compared wild-type control plants. study has advanced our understanding regulation provided useful information for improving