Influence of isopropylmalate synthase OsIPMS1 on seed vigour associated with amino acid and energy metabolism in rice

• DOI: 10.1111/pbi.12979
• Publication Date: 2018-06-27T09:24:54Z
• Authors: Yongqi He; Jinping Cheng; Ying He; Bin Yang; Yanhao Cheng; Can Yang; Hongsheng Zhang; Zhoufei Wang
• Keywords: 0301 basic medicine; 2. Zero hunger; Germination; Oryza; Gene Expression Regulation, Enzymologic; 03 medical and health sciences; Gene Expression Regulation, Plant; Leucine; Seedlings; Mutation; Seeds; Amino Acids; Energy Metabolism; Glycolysis; Research Articles; Acyltransferases; Plant Proteins

Seed vigour is an imperative trait for the direct seeding of rice. Isopropylmalate synthase (IPMS) catalyses committed step leucine (Leu) biosynthesis, but its effect on seed remains unclear. In this study, rice OsIPMS1 and OsIPMS2 was cloned, roles in were mainly investigated. catalyse Leu feedback inhibits their IPMS activities. Disruption resulted low under various conditions, which might be tightly associated with reduction amino acids germinating seeds. Eleven that stress tolerance, GA biosynthesis tricarboxylic acid (TCA) cycle significantly reduced osipms1 mutants compared those wide type (WT) during germination. Transcriptome analysis indicated a total 1209 differentially expressed genes (DEGs) altered osipms1a mutant WT at early germination stage, wherein most involved glycolysis/gluconeogenesis, protein processing, pyruvate, carbon, fructose mannose metabolism. Further confirmed regulation starch hydrolysis, glycolytic activity energy levels The effects priming mRNA used as biomarker to determine best stop time-point This study provides novel insights into function should have practical applications