FLOURY ENDOSPERM16 encoding a NAD‐dependent cytosolic malate dehydrogenase plays an important role in starch synthesis and seed development in rice

• DOI: 10.1111/pbi.13108
• Publication Date: 2019-03-12T09:05:30Z
• Authors: Xuan Teng; Mingsheng Zhong; Xiaopin Zhu; Chunming Wang; Yulong Ren; Yunlong Wang; Huan Zhang; Ling Jiang; Di Wang; Yuanyuan Hao; Mingming Wu; Jianping Zhu; Xin Zhang; Xiuping Guo; Yihua Wang; Jianmin Wan
• Keywords: Starch synthase; Malate dehydrogenase

Starch is the most important form of energy storage in cereal crops. Many key enzymes involved starch biosynthesis have been identified. However, molecular mechanisms underlying regulation are largely unknown. In this study, we isolated a novel floury endosperm rice (Oryza sativa) mutant flo16 with defective grain (SG) formation. The amylose content and amylopectin structure were both altered mutant. Map-based cloning complementation tests demonstrated that FLO16 encodes NAD-dependent cytosolic malate dehydrogenase (CMDH). ATP contents decreased mutant, resulting significant reductions activity synthesis-related enzymes. Our results indicated plays critical role redox homeostasis for compound SG formation subsequent endosperm. Overexpression significantly improved weight, suggesting possible application breeding. These findings provide insight into synthesis seed development rice.