CTP synthase is essential for early endosperm development by regulating nuclei spacing

• DOI: 10.1111/pbi.13644
• Publication Date: 2021-05-31T17:28:37Z
• Authors: Jinmi Yoon; Lae‐Hyeon Cho; Sung‐Ryul Kim; Win Tun; Xin Peng; Richa Pasriga; Sunok Moon; Woo‐Jong Hong; Hyeonso Ji; Ki‐Hong Jung; Jong‐Seong Jeon; Gynheung An
• Keywords: Wild type

Summary Cereal grain endosperms are an important source of human nutrition. Nuclear division in early endosperm development plays a major role determining seed size; however, this is not well understood. We identified the rice mutant endospermless 2 ( enl2 ), which shows defects stages development. These phenotypes arise from mutations OsCTPS1 that encodes cytidine triphosphate synthase (CTPS). Both wild‐type and were normal at 8 h after pollination (HAP). In contrast, 24 HAP, had approximately 10–16 clumped nuclei while increased number migrated to periphery. Staining microtubules HAP revealed evenly distributed by enl2‐2 tightly packed due their reduction microtubule association. addition, interacts with tubulins; thus, these observations suggest may be involved formation. transiently formed macromolecular structures during developmental stages, further supporting idea function as structural component Finally, overexpression weight promoting nuclear division, suggesting trait could used increase yield.