The GhMYB36 transcription factor confers resistance to biotic and abiotic stress by enhancing PR1 gene expression in plants

• DOI: 10.1111/pbi.13751
• Publication Date: 2021-11-23T09:56:41Z
• Authors: Tingli Liu; Tianzi Chen; Jialiang Kan; Yao Yao; Dongshu Guo; Yuwen Yang; Xitie Ling; Jinyan Wang; Baolong Zhang
• Keywords: Verticillium dahliae; Verticillium wilt; WRKY protein domain; MYB; Ectopic expression; Verticillium

Drought and Verticillium wilt disease are two main factors that limit cotton production, which necessitates the identification of key molecular switch to simultaneously improve resistance dahliae tolerance drought stress. R2R3-type MYB proteins could play such a role because their conserved functions in plant development, growth, metabolism regulation, however, till date gene conferring desired both biotic abiotic stresses has not been found cotton. Here, we describe GhMYB36, encoding protein Gossypium hirsutum, confers Verticilium Arabidopsis GhMYB36 was highly induced by PEG-simulated stress G. hirsutum. GhMYB36-silenced plants were more sensitive wilt. overexpression transgenic gave rise improved resistance. Transient expression fused GhMYB36-GFP tobacco cells able localize cell nucleus. In addition, RNA-seq analysis together with qRT-PCR validation overexpressing revealed significantly enhanced PR1 expression. Luciferase interaction assays indicated probably bound promoter activate its interaction, further verified Yeast one hybrid assay. Taken together, our results suggest as transcription factor is involved enhancing