Natural variation of HTH5 from wild rice, Oryza rufipogon Griff., is involved in conferring high‐temperature tolerance at the heading stage

• DOI: 10.1111/pbi.13835
• Publication Date: 2022-05-06T04:25:57Z
• Authors: Zhibin Cao; Huiwu Tang; Yaohui Cai; Bohong Zeng; Jialiang Zhao; Xiuying Tang; Ming Lu; Huimin Wang; Xuejing Zhu; Xiaofeng Wu; Linfeng Yuan; Jianlin Wan
• Keywords: Oryza rufipogon; Oryza; Genetically modified rice

Global warming is a major abiotic stress factor, which limit rice production. Exploiting the genetic basis of natural variation in heat resistance at different reproductive stages among diverse exotic Oryza germplasms can help breeding heat-resistant cultivars. Here, we identified stable quantitative trait locus (QTL) for tolerance heading stage on chromosome 5 (qHTH5) O. rufipogon Griff. The corresponding gene, HTH5, pertains to pyridoxal phosphate-binding protein PLPBP (formerly called PROSC) family, predicted encode phosphate homeostasis (PLPHP) localized mitochondrion. Overexpression HTH5 increased seed-setting rate plants under stage, whereas suppression resulted greater susceptibility stress. Further investigation indicated that reduces reactive oxygen species accumulation high temperatures by increasing heat-induced 5'-phosphate (PLP) content. Moreover, found two SNPs located promoter region are involved with its expression level and associated diversity. These findings suggest novel gene might have great potential value heightening on-going threat global warming.