The autotetraploid potato genome provides insights into highly heterozygous species

• DOI: 10.1111/pbi.13883
• Publication Date: 2022-06-29T16:20:46Z
• Authors: Fang Wang; Zhiqiang Xia; Meiling Zou; Long Zhao; Sirong Jiang; Yun Zhou; Chenji Zhang; Yongzhen Ma; Yuting Bao; Haihong Sun; Wenquan Wang; Jian Wang
• Keywords: Sequence assembly; Synteny

Summary Potato ( Solanum tuberosum L.) originated in the Andes and evolved its vegetative propagation strategy through short day‐dependent tuber development. Herein, we present a high‐quality, chromosome‐scale reference genome sequence of tetraploid potato cultivar. The total length this assembly was 2.67 Gb, with scaffold N50 contig sizes 46.24 2.19 Mb, respectively. In total, 1.69 Gb repetitive sequences were obtained de novo annotation, long terminal repeats main transposable elements. A 126 070 protein‐coding genes annotated, which 125 077 (99.21%) located on chromosomes. 48 chromosomes classified into four haplotypes. We annotated 31 506 homologous genes, including 5913 (18.77%) homologues, 11 103 (35.24%) three 12 177 (38.65%) two homologues 2313 (7.34%) one homologue. MLH3 , MSH6/7 RFC3 are involved mismatch repair pathway, found to be significantly expanded relative diploid genome. Genome‐wide association analysis revealed that cytochrome P450, flavonoid synthesis, chalcone enzyme, glycosyl hydrolase transferase correlated flesh colours 150 potatoes. This study provides valuable insights highly heterozygous autotetraploid may facilitate development tools for cultivar breeding further studies crops.