Profiling of phytohormone‐specific microRNAs and characterization of the miR160‐ARF1 module involved in glandular trichome development and artemisinin biosynthesis in Artemisia annua

• DOI: 10.1111/pbi.13974
• Publication Date: 2022-12-07T20:59:20Z
• Authors: Zhiying Guo; Kai Hao; Zongyou Lv; Luyao Yu; Qitao Bu; Junze Ren; Henan Zhang; Ruibing Chen; Lei Zhang
• Keywords: Artemisia annua; Trichome

Summary MicroRNAs (miRNAs) play crucial roles in plant development and secondary metabolism through different modes of sequence‐specific interaction with their targets. Artemisinin biosynthesis is extensively regulated by phytohormones. However, the function phytohormone‐responsive miRNAs artemisinin remains enigmatic. Thus, we combined analysis transcriptomics, small RNAs, degradome to generate a comprehensive resource for identifying key miRNA‐target circuits involved phytohormone‐induced process Artemisia annua . In total, 151 conserved 52 novel 4132 targets were determined. Based on differential expression analysis, miR160 was selected as potential miRNA synthesis. Overexpressing MIR160 significantly impaired glandular trichome formation suppressed A. , while repressing its resulted opposite effect, indicating that negatively regulates biosynthesis. RNA ligase‐mediated 5′ RACE transient transformation assays showed mediates cleavage Auxin Response Factor 1 ( ARF1 ) Furthermore, shown increase synthesis activating AaDBR2 expression. Taken together, our results reveal intrinsic link between miR160‐ARF1 module biosynthesis, may expedite innovation metabolic engineering approaches high stable production future.