Two tandem R2R3 MYB transcription factor genes cooperatively regulate anthocyanin accumulation in potato tuber flesh

• DOI: 10.1111/pbi.14602
• Publication Date: 2025-01-30T17:25:39Z
• Authors: Hui Du; Zefeng Zhai; Jin Pu; Jun Liang; Rongyan Wang; Zhong Zhang; Pei Wang; Yanhui Zhu; Lian Huang; Dawei Li; Kaiyuan Chen; Guangtao Zhu; Chunzhi Zhang
• Keywords: Flesh; MYB; Structural gene

Summary Anthocyanin biosynthesis and accumulation determines the colour of tuber flesh in potato ( Solanum tuberosum ) influences nutritional quality. However, regulatory mechanism behind anthocyanin remains unclear. In this study, we identified Pigmented Pf locus through a genome‐wide association study using 135 diploid landraces. Genome editing two tandem R2R3 MYB transcription factor genes, StMYB200 StMYB210 , within demonstrated that both genes are involved flesh. Molecular biochemical assays revealed promotes by directly binding to 1.7‐kb insertion present promoter, while also regulates its own expression. Furthermore, activated expression basic helix–loop–helix gene StbHLH1 interacted with regulate biosynthesis. An analysis promoter different accessions showed is associated potato. These findings reveal genetic molecular which provide an important reference for breeding new varieties colourful