User‐friendly markers linked to Fusarium wilt race 1 resistance Fw gene for marker‐assisted selection in pea

• DOI: 10.1111/pbr.12085
• Publication Date: 2013-09-04T07:19:22Z
• Authors: Soon Jae Kwon; Petr Smýkal; Jinguo Hu; Meinan Wang; Sung‐Jin Kim; Rebecca J. McGee; Kevin McPhee; Clarice J. Coyne
• Keywords: Marker-Assisted Selection; Molecular marker; Genetic linkage

Abstract Fusarium wilt is one of the most widespread diseases pea. Resistance to F usarium race 1 was reported as a single gene, Fw , located on linkage group III. The previously AFLP and RAPD markers linked have limited usage in marker‐assisted selection due their map distance phase. Using 80 8 recombinant inbred lines ( RIL s) derived from cross G reen A rrow × PI 179449, we amplified 72 polymorphic between resistant susceptible with target region polymorphism TRAP ) technique. Marker–trait association analysis revealed significant association. Five candidate were identified three converted into user‐friendly dominant SCAR markers. Forty‐eight pea cultivars known or phenotypes verified marker–trait These markers, Fw_Trap_480, Fw_Trap_340 Fw_Trap_220, are tightly only 1.2 c M away locus therefore ideal for selection. newly useful assist isolation resistance gene