Whole‐genome resequencing and transcriptome analysis provide insights on aphid‐resistant quantitative trait loci/genes in Sorghum bicolor

Bulked segregant analysis
DOI: 10.1111/pbr.12946 Publication Date: 2021-06-11T15:27:55Z
ABSTRACT
Abstract The sorghum aphid ( Melanaphis sacchari ) has emerged as a serious pest for Sorghum bicolor and major factor limiting its production worldwide. In this study, whole‐genome resequencing of resistant susceptible bulks from recombinant inbred line (RIL) population was conducted together with transcriptome analysis the parent to identify genes underlying resistance. NGS‐based bulked segregation (BSA), using total 920,389 genome‐wide high‐confidence markers, four QTLs, qtlMs‐6.1 , qtlMs‐6.2 qtlMs‐6.3 qtlMs‐6.4 were identified on chromosome 6. Based comparative analysis, 245 differentially expressed (DEGs,190 upregulated 55 downregulated) 576 DEGs (336 240 at 4 dpi in 407B (resistant) 7B (susceptible), respectively. enriched ‘DNA replication’, ‘flavone flavonol biosynthesis’ ‘flavonoid biosynthesis’, which are associated plant stress QTL‐seq study SNP marker (Chr6: 2686447C>G) that validated diverse panel could be used molecular marker‐assisted selection improve resistance sorghum.
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