The metabolic acclimation of Arabidopsis thaliana to arsenate is sensitized by the loss of mitochondrial LIPOAMIDE DEHYDROGENASE2, a key enzyme in oxidative metabolism

• DOI: 10.1111/pce.12187
• Publication Date: 2013-08-21T09:50:45Z
• Authors: WEIHUA CHEN; NICOLAS L. TAYLOR; YINGJUN CHI; A. HARVEY MILLAR; HANS LAMBERS; PATRICK M. FINNEGAN
• Keywords: 0303 health sciences; Arabidopsis Proteins; Acclimatization; Arabidopsis; Plant Roots; Mitochondria; 03 medical and health sciences; Phenotype; Gene Expression Regulation, Plant; Organ Specificity; Seedlings; Mutation; Metabolome; Arsenates; Promoter Regions, Genetic; Oxidation-Reduction; Dihydrolipoamide Dehydrogenase

Mitochondrial lipoamide dehydrogenase is essential for the activity of four mitochondrial enzyme complexes central to oxidative metabolism. The reduction in protein amount and caused by disruption LIPOAMIDE DEHYDROGENASE2 enhanced arsenic sensitivity Arabidopsis thaliana. Both arsenate arsenite inhibited root elongation, decreased seedling size increased anthocyanin production more profoundly knockout mutants than wild-type seedlings. Arsenate also stimulated lateral formation mutants. isolated mitochondria was sensitive arsenite, but not arsenate, indicating that could be mediator observed phenotypes. Steady-state metabolite abundances were only mildly affected mutation DEHYDROGENASE2. In contrast, induced remodelling pools associated with metabolism seedlings, an effect mutant, especially around containing dehydrogenase. These results indicate important determining Arabidopsis.