The terpene synthase gene family in Gossypium hirsutum harbors a linalool synthase GhTPS12 implicated in direct defence responses against herbivores

• DOI: 10.1111/pce.13088
• Publication Date: 2017-10-18T08:02:39Z
• Authors: Xin‐Zheng Huang; Yu‐Tao Xiao; Tobias G. Köllner; Wei‐Xia Jing; Jun‐Feng Kou; Jie‐Yin Chen; Dan‐Feng Liu; Shao‐Hua Gu; Jun‐Xiang Wu; Yong‐Jun Zhang; Yu‐Yuan Guo
• Keywords: Terpene; Gossypium

Abstract Herbivore‐induced terpenes have been reported to function as ecological signals in plant–insect interactions. Here, we showed that insect‐induced cotton volatile blends contained 16 terpenoid compounds with a relatively high level of linalool. The diversity terpene production is derived from large synthase (TPS) gene family. TPS family Gossypium hirsutum and raimondii consist 46 41 members, respectively. Twelve genes ( GhTPS4–15 ) could be isolated, protein expression Escherichia coli revealed catalytic activity for eight GhTPS. upregulation the majority these additionally supports herbivore‐induced biosynthesis. Furthermore, transgenic Nicotiana tabacum plants overexpressing GhTPS12 were generated, which produced amounts (3 S )‐linalool. In choice tests, female adults Helicoverpa armigera laid fewer eggs on compared non‐transformed controls. Meanwhile, Myzus persicae preferred feeding wild‐type leaves over plants. Our findings demonstrate transcript accumulation multiple mainly responsible cotton. Also, might play roles plant defence, particular, direct defence responses against herbivores.