The light‐induced transcription factor FtMYB116 promotes accumulation of rutin in Fagopyrum tataricum

• DOI: 10.1111/pce.13470
• Publication Date: 2018-10-30T14:22:52Z
• Authors: Dong Zhang; Chunli Jiang; Chenhao Huang; Dong Wen; Jiangnan Lu; Sha Chen; Tianyuan Zhang; Yuhua Shi; Jianping Xue; Wei Ma; Li Xiang; Wei Sun; Shilin Chen
• Keywords: Fagopyrum tataricum; Fagopyrum; MYB

Tartary buckwheat (Fagopyrum tataricum) not only provides a supplement to primary grain crops in China but also has high medicinal value, by virtue of its rich content flavonoids possessing antioxidant, anti-inflammatory, and anticancer properties. Light is an important environmental factor that can regulate the synthesis plant secondary metabolites. In this study, we treated tartary seedlings with different wavelengths light found red blue could increase expression genes involved flavonoid pathways. Through coexpression analysis, identified new MYB transcription (FtMYB116) be induced light. Yeast one-hybrid assays electrophoretic mobility shift assay showed FtMYB116 binds directly promoter region flavonoid-3'-hydroxylase (F3'H), transient luciferase activity indicated induce F3'H expression. After transforming into hairy roots buckwheat, observed significant increases rutin quercetin. Collectively, our results indicate promote seedlings; factor, FtMYB116, promotes accumulation via direct activation