Cell wall modification by the xyloglucan endotransglucosylase/hydrolase XTH19 influences freezing tolerance after cold and sub‐zero acclimation

• DOI: 10.1111/pce.13953
• Publication Date: 2020-11-15T05:05:31Z
• Authors: Daisuke Takahashi; Kim L. Johnson; Pengfei Hao; Tan Tuong; Alexander Erban; Arun Sampathkumar; Antony Bacic; David P. Livingston; Joachim Kopka; Takeshi Kuroha; Ryusuke Yokoyama; Kazuhiko Nishitani; Ellen Zuther; Dirk K. Hincha
• Keywords: Xyloglucan

Abstract Freezing triggers extracellular ice formation leading to cell dehydration and deformation during a freeze–thaw cycle. Many plant species increase their freezing tolerance exposure low, non‐freezing temperatures, process termed cold acclimation. In addition, mild temperatures after acclimation evokes further in (sub‐zero acclimation). Previous transcriptome proteome analyses indicate that wall remodelling may be particularly important for sub‐zero the present study, we used combination of immunohistochemical, chemical spectroscopic characterize walls Arabidopsis thaliana characterized mutant XTH19 gene, encoding xyloglucan endotransglucosylase/hydrolase (XTH). The showed reduced both acclimation, compared Col‐0 wild type, which was associated with differences composition structure. Most strikingly, immunohistochemistry 3D reconstruction centres rosette indicated epitopes xyloglucan‐specific antibody LM25 were highly abundant vasculature plants but absent mutant. Taken together, our data shed new light on potential roles increased observed low temperature