Multi‐omic dissection of the drought resistance traits of soybean landrace LX
Flavonoids
0301 basic medicine
2. Zero hunger
Glycine max
Propanols
Gene Expression Profiling
Reproducibility of Results
Secondary Metabolism
Germination
Genomics
15. Life on land
6. Clean water
Droughts
Anthocyanins
03 medical and health sciences
Phenotype
Quantitative Trait, Heritable
Gene Expression Regulation, Plant
Stress, Physiological
Metabolome
Metabolomics
Transcriptome
Transcription Factors
DOI:
10.1111/pce.14025
Publication Date:
2021-02-09T02:29:16Z
AUTHORS (16)
ABSTRACT
AbstractWith diverse genetic backgrounds, soybean landraces are valuable resource for breeding programs. Herein, we apply multi‐omic approaches to extensively characterize the molecular basis of drought tolerance in the soybean landrace LX. Initial screens established that LX performed better with PEG6000 treatment than control cultivars. LX germinated better than William 82 under drought conditions and accumulated more anthocyanin and flavonoids. Untargeted mass spectrometry in combination with transcriptomic analyses revealed the chemical diversity and genetic basis underlying the overall performance of LX landrace. Under control and drought conditions, significant differences in the expression of a suite of secondary metabolism genes, particularly those involved in the general phenylpropanoid pathway and flavonoid but not lignin biosynthesis, were seen in LX and William 82. The expression of these genes correlated with the corresponding metabolites in LX plants. Further correlation analysis between metabolites and transcripts identified pathway structural genes and transcription factors likely are responsible for the LX agronomic traits. The activities of some key biosynthetic genes or regulators were confirmed through heterologous expression in transgenic Arabidopsis and hairy root transformation in soybean. We propose a regulatory mechanism based on flavonoid secondary metabolism and adaptive traits of this landrace which could be of relevance to cultivated soybean.
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