Poplar acetylome profiling reveals lysine acetylation dynamics in seasonal bud dormancy release

• DOI: 10.1111/pce.14040
• Publication Date: 2021-03-06T07:44:07Z
• Authors: Xiaoli Liao; Yue Li; Zhenzhu Hu; Ying Lin; Bo Zheng; Jihua Ding
• Keywords: Isocitrate dehydrogenase

For perennials in boreal and temperate ecosystems, bud dormancy is crucial for survival harsh winter. Dormancy released by prolonged exposure to low temperatures followed reactive growth the spring. Lysine acetylation (Kac) one of major post-translational modifications (PTMs) that are involved plant response environmental signals. However, little information available on effects Kac modification release. Here, we report dynamics lysine acetylome hybrid poplar (Populus tremula × Populus alba) dormant buds. A total 7,594 acetyl-sites from 3,281 acetyl-proteins were identified, representing a large dataset plants. Of them, 229 proteins differentially acetylated during release mainly primary metabolic pathways. Site-directed mutagenesis enzymatic assays showed strongly modified activities two key enzymes metabolism, pyruvate dehydrogenase (PDH) isocitrate (IDH). We thus propose could be an important strategy reconfiguration processes In all, our results reveal importance provide new perspective understand molecular mechanisms seasonal trees.