Changing light promotes isoflavone biosynthesis in soybean pods and enhances their resistance to mildew infection

• DOI: 10.1111/pce.14128
• Publication Date: 2021-06-12T15:18:42Z
• Authors: Xiaoman Li; Caiqiong Yang; Jianhua Chen; Yuanyuan He; Juncai Deng; Congwei Xie; Xinli Xiao; Xiyang Long; Xiaoling Wu; Weiguo Liu; Junbo Du; Feng Yang; Xiaochun Wang; Taiwen Yong; Jing Zhang; Yushan Wu; Wenyu Yang; Jiang Liu
• Keywords: Jasmonic acid; Shading; Intercropping

Mildew severely reduces soybean yield and quality, pods are the first line of defence against pathogens. Maize-soybean intercropping (MSI) mildew incidence on pods; however, mechanism remains unclear. Changing light (CL) from maize shading is most important environmental feature in MSI. We hypothesized that CL affects isoflavone accumulation pods, affecting their disease resistance. In present study, treatments were applied to plants during different developmental stages according various environments under Chlorophyll fluorescence imaging (CFI) classical evaluation methods confirmed CL, especially vegetative stage (VS), enhanced pod resistance mildew. Further metabolomic analyses exogenous jasmonic acid (JA) biosynthesis inhibitor experiments revealed relationship between JA biosynthesis, which had a synergistic effect CL-treated VS promoted constitutive isoflavones upstream pathway, such as aglycones glycosides, pods. When infects endogenous signalling stimulated downstream inducible malonyl (MIF) glyceollin improve