Mechanisms of xylem hydraulic recovery after drought in Eucalyptus saligna

• DOI: 10.1111/pce.14265
• Publication Date: 2022-02-04T12:12:10Z
• Authors: Alice Gauthey; Jennifer M. R. Pe...; Rosana Lòpez; Madeline R. Carin...; Celia M. Rodrigue...; David T. Tissue; Belinda E. Medlyn; Tim J. Brodribb; Brendan Choat
• Keywords: 0301 basic medicine; Eucalyptus; 0303 health sciences; Water; X-Ray Microtomography; 15. Life on land; 6. Clean water; Droughts; Plant Leaves; 03 medical and health sciences; Xylem transpor; Xylem; Water relations; XXXXXX - Unknown

AbstractThe mechanisms by which woody plants recover xylem hydraulic capacity after drought stress are not well understood, particularly with regard to the role of embolism refilling. We evaluated the recovery of xylem hydraulic capacity in young Eucalyptus saligna plants exposed to cycles of drought stress and rewatering. Plants were exposed to moderate and severe drought stress treatments, with recovery monitored at time intervals from 24 h to 6 months after rewatering. The percentage loss of xylem vessels due to embolism (PLV) was quantified at each time point using microcomputed tomography with stem water potential (Ψx) and canopy transpiration (Ec) measured before scans. Plants exposed to severe drought stress suffered high levels of embolism (47.38% ± 10.97% PLV) and almost complete canopy loss. No evidence of embolism refilling was observed at 24 h, 1 week, or 3 weeks after rewatering despite rapid recovery in Ψx. Recovery of hydraulic capacity was achieved over a 6‐month period by growth of new xylem tissue, with canopy leaf area and Ec recovering over the same period. These findings indicate that E. saligna recovers slowly from severe drought stress, with potential for embolism to persist in the xylem for many months after rainfall events.