ABA biosynthesis gene OsNCED3 contributes to preharvest sprouting resistance and grain development in rice

• DOI: 10.1111/pce.14480
• Publication Date: 2022-11-02T03:59:37Z
• Authors: Yi Chen; Zhipan Xiang; Min Liu; Siyao Wang; Lin Zhang; Dan Cai; Yuan Huang; Dandan Mao; Jun Fu; Liangbi Chen
• Keywords: Preharvest; Gibberellic acid; Sprouting; Caryopsis; Zeatin

Preharvest sprouting (PHS) is an unfavorable trait in cereal crops and causes serious yield loss. However, the molecular mechanism underlying PHS remains largely elusive. Here, we identified a member of 9-cis-epoxycarotenoid dioxygenase family, OsNCED3, which regulates grain development rice (Oryza sativa L.). OsNCED3 encodes chloroplast-localized abscisic acid (ABA) biosynthetic enzyme highly expressed embryo developing seeds. Disruption by CRISPR/Cas9-mediated mutagenesis led to lower ABA higher gibberellic (GA) levels (thus skewed ABA/GA ratio) embryo, promoting embryos growth breaking seed dormancy before maturity harvest, thus decreased enhanced rice. overexpression resistance regulating proper ratio embryo. Intriguingly, resulted increased size weight, whereas disruption function weight. Nucleotide diversity analyses suggested that may be selected during japonica populations adaptation germination. Taken together, have new OsNCED regulator involved development, provide potential target gene for improving