Elevated CO2 mitigates the impact of drought stress by upregulating glucosinolate metabolism in Arabidopsis thaliana

• DOI: 10.1111/pce.14521
• Publication Date: 2022-12-21T07:58:16Z
• Authors: Hamada AbdElgawad; Gaurav Zinta; Johann Hornbacher; Jutta Papenbrock; Marios N. Markakis; Han Asard; Gerrit T. S. Beem...
• Keywords: Drought Tolerance; Drought stress; Brassicaceae

Abstract Elevated CO 2 (eCO ) reduces the impact of drought, but mechanisms underlying this effect remain unclear. Therefore, we used a multidisciplinary approach to investigate interaction drought and eCO in Arabidopsis thaliana leaves. Transcriptome subsequent metabolite analyses identified strong induction aliphatic glucosinolate (GL) biosynthesis as main drought‐stressed results highlighted upregulation ABI5 downregulation WRKY6 3 transcription factors (TF), known enhance inhibit expression genes regulating GL (e.g., MYB28 29 TFs), respectively. In addition, positively regulated by MYB28/29 increasing accumulation precursors. To test role GLs stress‐mitigating , investigated genetic perturbations biosynthesis. Overexpression MYB28, 76 improved tolerance inducing stomatal closure maintaining plant turgor, whereas loss cyp79f reduced decreased tolerance. Overall, crucial metabolism stress mitigation could be beneficial trait overcome future climate challenges.