GhbZIP30‐GhCCCH17 module accelerates corm dormancy release by reducing endogenous ABA under cold storage in Gladiolus

• DOI: 10.1111/pce.14595
• Publication Date: 2023-05-02T07:16:11Z
• Authors: Jia‐hui Liang; Jing‐ru Li; Chang Liu; Wen‐qiang Pan; Wen‐jing Wu; Wen‐jing Shi; Lu‐jia Wang; Ming‐fang Yi; Jian Wu
• Keywords: Gladiolus; Chromatin immunoprecipitation; Corm

Abstract Gladiolus hybridus is one of the most popular flowers worldwide. However, its corm dormancy characteristic largely limits off‐season production. Long‐term cold treatment (LT), which increases sugar content and reduces abscisic acid (ABA), an efficient approach to accelerate release (CDR). Here, we identified a GhbZIP30‐ GhCCCH17 module that mediates antagonism between sugars ABA during CDR. We showed promoted CDR by reducing levels in Gladiolus. Our data demonstrated GhbZIP30 transcription factor directly binds zinc finger promoter activates transcription, confirmed yeast one‐hybrid, dual‐luciferase (Dual‐LUC), chromatin immunoprecipitation‐quantitative PCR (ChIP‐qPCR) electrophoretic mobility shift assay (EMSA). transcriptional activator, nuclear localisation altered surcose cytokinin treatments. Both positively respond LT, sugars, Silencing or resulted delayed regulating metabolic genes, while their overexpression Taken together, propose involved cold‐ glucose‐induced genes.