A transcriptional regulation of ERF15 contributes to ABA‐mediated cold tolerance in tomato

• DOI: 10.1111/pce.14816
• Publication Date: 2024-01-15T07:49:22Z
• Authors: Chaoyi Hu; Mengqi Wang; Changan Zhu; Shaofang Wu; Jiajia Li; Jingquan Yu; Zhangjian Hu
• Keywords: Cold stress; Cold tolerance; Fluridone; Cold sensitivity; Transcription

Abstract Cold stress is a major meteorological threat to crop growth and yield. Abscisic acid (ABA) plays important roles in plant cold tolerance by activating the expression of cold‐responsive genes; however, underlying transcriptional regulatory module remains unknown. Here, we demonstrated that cold‐ ABA‐responsive transcription factor ETHYLENE RESPONSE FACTOR 15 (ERF15) positively regulates ABA‐mediated tomato. Exogenous ABA treatment significantly enhanced wild‐type tomato plants but failed rescue erf15 mutants from stress. Transcriptome analysis showed ERF15 was associated with factors such as CBF1 WRKY6 . Further RT‐qPCR assays confirmed ABA‐induced increased transcripts suppressed when were subjected treatment. Moreover, yeast one‐hybrid assays, dual‐luciferase electrophoretic mobility shift activated binding their promoters. Silencing or decreased tolerance. Overall, our study identified role conferring expression. This not only broadens knowledge mechanism also highlights an ideal target gene for cold‐tolerant breeding.