Single‐cell transcriptome landscape elucidates the cellular and developmental responses to tomato chlorosis virus infection in tomato leaf

• DOI: 10.1111/pce.14906
• Publication Date: 2024-04-15T12:09:33Z
• Authors: Hao Yue; Gong Chen; Zhuo Zhang; Zhaojiang Guo; Zhanhong Zhang; Songbai Zhang; Ted C. J. Turlings; Xuguo Zhou; Jing Peng; Yang Gao; Deyong Zhang; Xiaobin Shi; Yong Liu
• Keywords: Chlorosis

Plant viral diseases compromise the growth and yield of crop globally, they tend to be more serious under extreme temperatures drought climate changes. Currently, regulatory dynamics during plant development in response virus infection at cell level remain largely unknown. In this study, single-cell RNA sequencing on 23 226 individual cells from healthy tomato chlorosis virus-infected leaves was established. The specific expression epigenetic landscape each type stage were depicted. Notably, mesophyll showed a rapid function transition leaves, which is consistent with pathological changes such as thinner decreased chloroplast lamella samples. Interestingly, F-box protein SKIP2 identified play pivotal role chlorophyll maintenance plants. Knockout SlSKIP2 greener leaf state before after infection. Moreover, we further demonstrated that located cytomembrane nucleus directly regulated by ERF4. conclusion, detailed insights into responses infections cellular level, our study provides genetic framework gene reference plant-virus interaction breeding future research.