Establishment of a mouse model for post‐inflammatory hyperpigmentation

• DOI: 10.1111/pcmr.12911
• Publication Date: 2020-07-05T06:38:50Z
• Authors: Shoko Nakano; Yuko Abe; Kimiko Nakajima; Shigetoshi Sano; Osamu Yamamoto; Kazumasa Wakamatsu; Shosuke Ito; Masahiro Hayashi; Tamio Suzuki
• Keywords: Inflammation; Male; Melanins; Mice, Transgenic; 3. Good health; Disease Models, Animal; Mice; 03 medical and health sciences; 0302 clinical medicine; Hyperpigmentation; Animals; Humans; Female; Epidermis

Post-inflammatory hyperpigmentation (PIH) is a common cutaneous condition that can cause disfigured appearance. However, the pathophysiology of PIH remains poorly understood, at least in part, because an appropriate animal model for research has not been established. In order to analyze pathomechanism PIH, we successfully induced hairless version transgenic mice (hk14-SCF Tg/HRM) have human-type epidermis containing melanin by repeated hapten application 2,4-dinitrofluorobenzene. Histopathologic observation showed epidermal hyperplasia, predominant infiltrations inflammatory cells, and melanin-containing cells dermis just after elicitation atopic dermatitis-like condition. At week 2, findings were similar characteristics is, increase without spongiosis or liquid degeneration dermal melanophages. Dynamic analysis remained longer duration than epidermis. Furthermore, immunohistochemical staining revealed majority positive anti-CD68 antibody, but negative anti-F4/80 antibody. These data suggest novel treatments should be targeted against macrophages eventually lead development new treatment modalities.