Protection and immunological study on two tetraspanin‐derived vaccine candidates against schistosomiasis japonicum

• DOI: 10.1111/pim.12338
• Publication Date: 2016-05-18T12:55:31Z
• Authors: L. Chen; Y. Chen; D. Zhang; M. Hou; B. Yang; F. Zhang; W. Zhang; X. Luo; M. Ji; G. Wu
• Keywords: Tetraspanin; Schistosoma; CD81

Tetraspanins (TSPs) are proteins found on the surface of helminth parasites genus Schistosoma and regarded as potentially protective antigens. The large extracellular loop mansoni tetraspanin-2, Sm-TSP-2, when fused to a thioredoxin partner formulated with Freund's adjuvants, has been shown be an efficacious vaccine against murine schistosomiasis. It is well recognized that CD4(+) T-cell-dependent immunity might play important role schistosomes; however, contribution CD8(+) T cells multicellular pathogen still uncertain. exogenous protein-pulsed dendritic (DCs) can easily activate response, while response was relatively difficult induced. In this study, we evaluated immunogenicity TSP2HD antigen (hydrophilic domain S. japonicum tetraspanin-2) TAT (the protein transduction HIV-1)-coupled protein. As TAT-fused could promote major histocompatibility complex class I-dependent presentation in vitro, TAT-TSP2HD-pulsed DCs induced stronger proliferation schistosome-specific compared incubated alone. Vaccination vivo improve disease outcome japonicum-infected mice slightly superior vaccination treated TSP2HD. summary, these data showed fusion help Th1 provide part protection schistosome.