Evaluation of gene expression levels for cytokines in ocular toxoplasmosis

• DOI: 10.1111/pim.12462
• Publication Date: 2017-08-24T12:39:02Z
• Authors: M. M. Maia; C. S. Meira‐Strej...; V. L. Pereira‐Chi...; D. D. C. de Hippó...; V. O. Silva; C. C. Brandão de ...; F. B. Frederico; R. C. Siqueira; L. C. de Mattos
• Keywords: Toxoplasmosis

This study evaluated levels for mRNA expression of 7 cytokines in ocular toxoplasmosis. Peripheral blood mononuclear cells (PBMC) patients with toxoplasmosis (OT Group, n = 23) and chronic individuals (CHR 9) were isolated stimulated vitro T. gondii antigen. Negative controls (NC) constituted PBMC samples from seronegative was determined by qPCR. Results showed a significant increase antigen PBMCs derived OT Group expressing IL-6 (at P < .005 .0005 CHR NC groups, respectively), IL-10 respectively) TGF-β .005) group. TNF-α IL-12 also upregulated compared to individuals, although without statistical significance. Additionally, IL-27 IFN-γ comparison individuals. Differences between groups statistically at .05 .0005, respectively.