Two guard cell mitogen‐activated protein kinases, MPK9 and MPK12, function in methyl jasmonate‐induced stomatal closure in Arabidopsis thaliana

• DOI: 10.1111/plb.12321
• Publication Date: 2015-02-19T11:01:06Z
• Authors: Md. A. R. Khokon; M. A. Salam; F. Jammes; W. Ye; M. A. Hossain; M. Uraji; Y. Nakamura; I. C. Mori; J. M. Kwak; Y. Murata
• Keywords: Methyl jasmonate; Wild type

Abstract Methyl jasmonate (Me JA ) and abscisic acid ( ABA signalling cascades share several components in guard cells. We previously showed that two cell‐preferential mitogen‐activated protein kinases MAPK s), MPK 9 12 , positively regulate Arabidopsis thaliana . In this study, we examined whether these MAP function Me using genetic mutants for combined with a pharmacological approach. induced stomatal closure mpk9‐1 mpk12‐1 single as well wild‐type plants, but not double mutants. Consistently, the MAPKK inhibitor PD 98059 inhibited ‐induced plants. elicited reactive oxygen species ROS production cytosolic alkalisation cells of mpk9‐1, mutants, Furthermore, triggered elevation Ca 2+ concentration ([Ca ] cyt mutant Activation S‐type anion channels by was impaired Together, results indicate upstream channel activation downstream production, [Ca cell signalling, suggesting are key regulators mediating both