Interactive effects of hydrogen sulphide and silicon enhance drought and heat tolerance by modulating hormones, antioxidant defence enzymes and redox status in barley (Hordeum vulgare L.)

• DOI: 10.1111/plb.13374
• Publication Date: 2021-12-08T17:15:50Z
• Authors: R. Naz; F. Gul; S. Zahoor; A. Nosheen; H. Yasmin; R. Keyani; M. Shahid; M. N. Hassan; M. H. Siddiqui; S. Batool; Z. Anwar; N. Ali; T. H. Roberts
• Keywords: Thermotolerance; 2. Zero hunger; 0301 basic medicine; Silicon; Hordeum; 15. Life on land; Antioxidants; Hormones; 6. Clean water; Droughts; 03 medical and health sciences; Stress, Physiological; 13. Climate action; Hydrogen Sulfide; Reactive Oxygen Species; Oxidation-Reduction

Abstract

Recent changes in climate have reduced crop productivity throughout much of the world. Drought and heat stress, particularly in arid and semi‐arid regions, have seriously affected barley production. This study explored the separate and interactive effects of silicon (Si) and hydrogen sulphide (H2S) on plant growth and mitigation of the adverse effects of heat stress (DS) and drought stress (HS) in a barley pot experiment. The impacts of simultaneous DS + HS were more severe than individual stresses due to increased ROS production, malondialdehyde (MDA) content and higher electrolyte leakage (EL), thereby leading to reduced water, protein and photosynthetic pigment content. Exogenously applied Si and H2S alleviated the DS‐, HS‐ and DS + HS‐induced effects on barley by reducing ROS production, MDA and EL. A single application of H2S or Si + H2S increased plant biomass under all stress conditions, which can be ascribed to higher Si accumulation in barley shoots. A single application of Si or H2S significantly increased plant biomass. However, Si + H2S was the most effective treatment for metabolite accumulation and elevating activity of antioxidant enzymes to prevent toxicity from oxidative stress. This treatment also modulated osmolyte content, enhanced antioxidant activity and regulated the stress signalling‐related endogenous hormones, abscisic acid (ABA) and indole acetic acid (IAA). Exogenous treatments regulated endogenous H2S and Si and resulted in higher tolerance to individual and combined drought and heat stress in barley.