Using high‐throughput sequencing in support of a plant health outbreak reveals novel viruses in Ullucus tuberosus (Basellaceae)

• DOI: 10.1111/ppa.12962
• Publication Date: 2018-11-18T10:13:28Z
• Authors: A. Fox; A. R. Fowkes; A. Skelton; V. Harju; A. Buxton‐Kirk; M. Kelly; S. M. D. Forde; H. Pufal; C. Conyers; R. Ward; R. Weekes; N. Boonham; I. P. Adams
• Keywords: Cucumovirus

Ullucus tuberosus (ulluco) is a tuber‐forming species that has become novel crop in highland and temperate maritime climates. Eight viruses have been previously reported infecting , including Andean potato latent virus ( APLV ), quarantine within the European Union. No reference sequences published for described from U . Plants grown UK internet trade were tested presence of using ELISA real‐time RT ‐ PCR positive results obtained multiple other viruses. A similar suite was detected at second outbreak site linked to horticultural trade. Virus identification by high‐throughput sequencing HTS ) ribosomal RNA rRNA )‐depleted total approach. Analysis viral contigs indicated several closely related to, but not consistent with, Further confirmatory testing two tymoviruses, tentatively named tymovirus 1 2, more each (85% identity), than or mild mosaic (63–66% identity). could be confirmed either tobamovirus (Ullucus 1) only initial site. polerovirus distinct genotype Papaya both sites. Deploying during plant health demonstrates potential this approach give rapid, accurate diagnosis.