Sugarcane mild mosaic virus: The rediscovery of an identified but unrecognized virus

• DOI: 10.1111/ppa.13654
• Publication Date: 2022-10-01T04:39:06Z
• Authors: Dimitre Mollov; Denis Filloux; Samuel Grinstead; Stephen Bolus; Lisa Claude; Emanuel Fernandez; Charlotte Julian; Thierry Candresse; Ben Lockhart; Philippe Roumagnac; Philippe Rott; Jean Heinrich Dau...
• Keywords: Amplicon; Sanger sequencing; Virus classification

Abstract The complete genome sequence of a previously uncharacterized sugarcane‐infecting virus the genus Ampelovirus was obtained by high‐throughput sequencing (HTS) and rapid amplification cDNA ends. This consists 13,114 nucleotides (nt) harbours seven open reading frames. Phylogenetic pairwise identity analyses with or near‐complete sequences 16 isolates revealed that they all belong to new species, family Closteroviridae . These were distributed in three phylogenetic groups potential recombinant events identified for only one these isolates. An immunocapture‐reverse transcription‐PCR (IC‐RT‐PCR) assay developed antibodies produced against clostero‐like had not yet been recognized International Committee on Taxonomy Viruses but which name sugarcane mild mosaic (SCMMV) proposed. ampelovirus detected IC‐RT‐PCR, Sanger‐sequenced amplicons confirmed based HTS data. SCMMV 65 350 samples quarantined germplasm using RT‐PCR targeting hsp70 gene. originated from Africa, Caribbean, North America, South America South‐east Asia, thus revealing is present many sugarcane‐growing regions world. reported here represent first genomic SCMMV. Furthermore, newly diagnostic will facilitate understanding epidemiology help improve management practices.