Characterization of virus species associated with sweet potato virus disease in Costa Rica and riboprobe development for its rapid detection

• DOI: 10.1111/ppa.14027
• Publication Date: 2024-11-03T10:34:26Z
• Authors: Daniela Méndez‐Na...; Randall Chacón‐Ce...; Carlos Alvarado‐U...; José Roberto Cama...; Jesús Ángel Sánch...; Luis Alvarado‐Mar...
• Keywords: Riboprobe

Abstract Sweet potato ( Ipomoea batatas ) is a crucial crop for food security and economic stability in many regions, including Costa Rica. This study aimed to assess the prevalence genetic diversity of two major viruses associated with sweet virus disease (SPVD) Rica: feathery mottle (SPFMV) chlorotic stunt (SPCSV). A total 50 leaf samples displaying typical viral symptoms were collected from potato‐growing regions. Using multiplex reverse transcription (RT)‐PCR, SPFMV was detected 82% SPCSV 52%, coinfection rate 48%. Genetic analysis revealed that isolates belonged predominantly phylogenetic group B, while clustered West African (WA) strain. The low within these populations suggests limited number initial infection events followed by local spread. Riboprobe technology evaluated as an alternative RT‐PCR detection. While riboprobes demonstrated sensitivity comparable RT‐PCR, showed slightly lower sensitivity, particularly when using crude extracts. Despite this, offer significant advantages terms cost ease use large‐scale screening. Our findings underscore need robust diagnostic management strategies control SPVD highlight importance continuous monitoring research mitigate impact diseases on production These insights contribute global understanding support collaborative efforts plant pathology research.