A model system to study the lignification process inEucalyptus globulus

• DOI: 10.1111/ppl.12152
• Publication Date: 2014-01-20T15:48:45Z
• Authors: Pedro Araújo; Igor Cesarino; Juliana Lischka S...; Ilse Fernanda Fer...; Eduardo Kiyota; Alexandra Christi...; Adriana Franco Pa...; Paulo Mazzafera
• Keywords: Eucalyptus globulus; Lignocellulosic Biomass

Recalcitrance of plant biomass is closely related to the presence phenolic heteropolymer lignin in secondary cell walls, which has a negative effect on forage digestibility, biomass-to-biofuels conversion and chemical pulping. The genus Eucalyptus main source wood for pulp paper industry. However, when compared model plants such as Arabidopsis thaliana poplar, relatively little known about biosynthesis only few genes were functionally characterized. An efficient, fast inexpensive vitro system was developed study lignification globulus evaluate potential role candidate this biological process. Seedlings grown four different conditions, or absence light with without sucrose growth medium, several aspects metabolism evaluated. Our results showed that and, lesser extent, induced biosynthesis, followed by changes S/G ratio, oligomers accumulation gene expression. In addition, higher total peroxidase activity differential isoperoxidase profile observed seedlings sucrose. Peptide sequencing allowed identification differentially expressed peroxidases, can be considered class III peroxidases involved polymerization E. globulus.