Cloning and characterization of a novel Nicotiana tabacumABC transporter involved in shoot branching

• DOI: 10.1111/ppl.12267
• Publication Date: 2014-08-29T12:16:54Z
• Authors: Xiaodong Xie; Genhong Wang; Ling Yang; Tingcai Cheng; Junping Gao; Yuqian Wu; Qingyou Xia
• Keywords: Expression cloning; Cloning (programming)

The ATP-binding cassette (ABC) superfamily is a large protein family with diverse physiological functions in all kingdoms of life. One distinguished subfamily, the pleiotropic drug resistance (PDR) transporters, has only been identified plants and fungi. Here, we Nicotiana tabacum PDR gene, NtPDR6, which homolog Petunia hybrida PDR1. full-length cDNA NtPDR6 had 4482-bp open reading frame encoding full-size ABC transporter 1493 amino acids. Sequence comparison showed that high homology plant proteins. was strongly induced by phosphate starvation as well 1-naphthalene acetic acid. Tissue expression pattern analysis detected surveyed tissues but preferentially roots. We cloned 1.3-kb promoter found there one response-related element Pho-like several root-specific expression-related elements rootmotiftapox1 promoter. A tissue-specific promoter-β-glucuronidase dominantly observed subepidermal cells elongation zone lateral RNA interference technology used to knock down expression, significantly increased branching phenotype knockdown plants. These data suggest plays key role regulation shoot processes.