Redox regulation of free amino acid levels in Arabidopsis thaliana

• DOI: 10.1111/ppl.12510
• Publication Date: 2016-09-08T08:56:40Z
• Authors: Zsolt Gulyás; Livia Simon‐Sarkadi; Eszter Badics; Aliz Novák; Zsuzsanna Mednyán...; Gabriella Szalai; Gábor Galiba; Gábor Kocsy
• Keywords: 2. Zero hunger; 0301 basic medicine; 0303 health sciences; Arabidopsis Proteins; Arabidopsis; Ascorbic Acid; Hydrogen Peroxide; Glutathione; Oxidative Stress; 03 medical and health sciences; Gene Expression Regulation, Plant; Stress, Physiological; Mutation; QK10 Plant physiology / növényélettan; Amino Acids; Oxidation-Reduction

Abiotic stresses induce oxidative stress, which modifies the level of several metabolites including amino acids. The redox control free acid profile was monitored in wild-type and ascorbate or glutathione deficient mutant Arabidopsis thaliana plants before after hydroponic treatment with various agents. Both mutations treatments modified size state (AsA) and/or (GSH) pools. total content increased by AsA, GSH H2 O2 all three genotypes a very large (threefold) increase observed GSH-deficient pad2-1 compared untreated plants. Addition reduced ratio acids belonging to glutamate family on scale relative amount non-proteinogenic latter change because alpha-aminoadipate, an inhibitor glutamatic (Glu) transport. Most proline (Pro) content, effect due activation genes involved Pro synthesis. Although studied compounds influenced mostly positive, close (r > 0.9) correlation exists between these parameters, special regulatory role could be presumed its more powerful effect. This may originate from thiol/disulphide conversion (de)glutathionylation enzymes participating metabolism.