Nitric oxide is involved in methane‐induced adventitious root formation in cucumber

• DOI: 10.1111/ppl.12531
• Publication Date: 2016-11-24T15:22:44Z
• Authors: Fang Qi; Zhixin Xiang; Ninghai Kou; Weiti Cui; Daokun Xu; Ren Wang; Dan Zhu; Wenbiao Shen
• Keywords: Organogenesis; Sodium nitroprusside; Lateral root

Our previous studies revealed that methane (CH4 ) induces adventitious rooting in cucumber. However, the corresponding molecular mechanism is still elusive. In this work, we discovered CH4 triggered accumulation of nitric oxide (NO) and thereafter cucumber rooting, mimicking inducing effects sodium nitroprusside (SNP) NONOate (two NO-releasing compounds). Above mentioned responses were sensitive to NO scavenger(s), showing root development respectively impaired. Inhibitor test biochemical analysis suggested endogenous mainly produced by mammalian synthase-like enzyme diamine oxidases (DAO), might be required for formation elicited . Molecular evidence confirmed -mediated induction several marker genes responsible development, including CsDNAJ-1, CsCDPK1, CsCDPK5, cell division-related gene CsCDC6, two auxin signaling genes, CsAux22D-like CsAux22B-like, was casually dependent on signaling. The possible involvement S-nitrosylation during preliminarily illustrated. Taken together, through pharmacological, anatomical approaches, it involved -induced -eliciated NO-targeted proteins partially modulated at transcriptional post-translational levels. work may increase understanding mechanisms underlying -elicited organogenesis higher plants.