Effect of temperature on the Arabidopsis cryptochrome photocycle

• DOI: 10.1111/ppl.13365
• Publication Date: 2021-02-14T04:40:39Z
• Authors: Marootpong Pooam; Nykiera Dixon; Michael Hilvert; Peter Misko; Kristy Waters; Nathalie Jourdan; Soria Drahy; Stephen Mills; Dorothy Engle; Justin Link; Margaret Ahmad
• Keywords: Photolyase

Abstract Cryptochromes are blue light‐absorbing photoreceptors found in plants and animals with many important signalling functions. These include control of plant growth, development, the entrainment circadian clock. Plant cryptochromes have recently been implicated adaptations to temperature variation, including compensation However, effect directly on photochemical properties cryptochrome photoreceptor remains unknown. Here we show that response light purified Arabidopsis Cry1 Cry2 proteins was significantly altered by temperature. Spectral analysis at 15°C showed a pronounced decrease flavin reoxidation rates from biologically active, light‐induced (FADH°) state inactive (FADox) resting redox as compared ambient (25°C) This result indicates low temperatures, concentration active FADH° form Cry is increased , leading counterintuitive prediction there should be an biological activity lower temperatures. confirmed using C‐terminal phosphorylation direct assay for activation vivo . We conclude enhanced function consistent modulation photocycle.