Genome‐wide transcriptional responses to water deficit during seed development in Pisum sativum, focusing on sugar transport and metabolism

• DOI: 10.1111/ppl.14062
• Publication Date: 2023-11-10T09:23:17Z
• Authors: Amélie Morin; Benoit Porcheron; Gatepe Cedoine Ko...; Bouziane Moumen; Cécile Vriet; Laurence Maurousset; Rémi Lemoine; Nathalie Pourtau; Joan Doidy
• Keywords: Carbohydrate Metabolism

Abstract Agriculture is particularly impacted by global changes, drought being a main limiting factor of crop production. Here, we focus on pea ( Pisum sativum ), model legume cultivated for its seed nutritional value. A water deficit (WD) was applied during early reproductive phase, harvesting plant organs at two key developmental stages, either the embryonic or seed‐filling stages. We combined phenotypic, physiological and transcriptome analyses to better understand adaptive response drought. First, showed that apical growth arrest major phenotypic indicator stress. Sugar content also greatly impacted, especially leaf fructose starch contents. Our RNA‐seq analysis identified 2001 genes regulated WD in leaf, 3684 root 2273 seed, while only 80 were seed‐filling. Hence, large transcriptional reprogramming occurred seeds stage, but no longer later stage filling. Biological processes involved regulation, carbon transport metabolism both source sink organs, as illustrated expression encoding transcription factors, sugar transporters enzymes synthesis pathway. then looked transcriptomic changes development, highlighting transition from monosaccharide utilization sucrose feeding pathway stage. Altogether, our study presents an integrative picture development genome‐wide level.