The Glycine soja cytochrome P450 gene GsCYP82C4 confers alkaline tolerance by promoting reactive oxygen species scavenging

Glycine soja Wild type
DOI: 10.1111/ppl.14252 Publication Date: 2024-03-21T07:19:58Z
ABSTRACT
Abstract Recent studies have demonstrated the crucial role of Cytochrome P450 enzymes (CYPs) in production secondary metabolites, phytohormones and antioxidants plants. However, their functional characterization specifically under alkaline stress remains elusive. CYP82C4 was key gene screened from a family wild soybean CYPs our previous studies. The aim this present study to clone Glycine soja GsCYP82C4 characterize its functions Arabidopsis max. results showed that displayed high expression different plant tissues at mature stages compared young stages. Further, higher temporal noted 6, 12 24 h time points after alkali treatment leaves roots. In addition, overexpression improved tolerance via increased root lengths fresh biomass strengthened antioxidant defense system reduction superoxide radicals transgenic lines type (WT) atcyp82c4 mutants. levels stress‐related marker genes were up‐regulated OX stress. analysis better hairy growth, weight, enzyme activities reduced lipid peroxidation rates WT (K599) line. total, positive roles both max alleviate altering abundance responsive genes, stronger roots, as well radicals.
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