Evaluation of in vitro culture systems for goat preantral follicles using reused ovaries from reproductive biotechniques: An alternative to maximize the potential of reproduction

• DOI: 10.1111/rda.13377
• Publication Date: 2018-11-16T19:25:54Z
• Authors: Amanda Michelle t...; Ana Flávia Bezerr...; Rodrigo Tenório P...; Hudson Henrique V...; Davide Rondina; José Ricardo Figu...; Deborah de Melo M...
• Keywords: Microdissection; Antral follicle

This study aimed to examine the in vitro culture of secondary preantral follicles, using reused ovaries, compare both 2D and 3D methods system medium replacement. Twenty-five pairs ovaries from mixed-breed goats were used for experiment. Follicular puncture antral follicles was performed production. After this procedure, submitted a microdissection procedure. The isolated randomly divided into three treatments: (a) Two-dimensional with partial replacement during (2D PR), (b) Three-dimensional addition (3D AD) (c) PR). period 18 days. All treatments at end (18 days) presented follicular survival rate which ranged 59% 70%, demonstrating that it possible perform an experiment had previously been another reproductive biotechnique. AD treatment showed percentage diameter higher than PR treatment, however, did not differ treatment. In conclusion, experiments employing use can be success after have follicles. Moreover, three-dimensional is recommended since more practical financially feasible.