Catalase and superoxide dismutase supplementation to the diluent for cryopreservation of collared peccary semen

• DOI: 10.1111/rda.14536
• Publication Date: 2024-01-31T11:23:31Z
• Authors: Samara Sandy Jerô...; Romário Parente d...; Andréia Maria da ...; Tayná Moura Matos; Andreza Vieira Br...; João Batista Frei...; José Rodolfo Lope...; Alexsandra Fernan...; Alexandre Rodrigu...
• Keywords: Diluent; Semen cryopreservation

Abstract We evaluated the effects of supplementation with catalase (CAT) and superoxide dismutase (SOD) antioxidants, isolated or combined, on freezability collared peccary sperm. Semen aliquots from ten individuals were diluted in Tris‐yolk without presence antioxidants CAT (200 400 IU/mL) SOD (150 300 alone combination (CAT: 200 IU/mL + SOD: 150 IU/mL), then cryopreserved liquid nitrogen (−196°C). After 1 week, samples thawed for kinetic parameters, membrane functionality integrity, mitochondrial activity, chromatin sperm morphology, cell binding capacity intracellular oxidative levels. Regardless use treatments did not differ any except treatment SOD, which showed highest percentage static cells (69.1 ± 4.4%) when compared to group antioxidant (51.6 3.3%). In general, all maintained around 50% functional membranes, 40% intact membranes more than 99% condensed chromatin, as well 70% morphologically normal cells. Regarding capacity, minimum maximum values found ranged 33.8 194 spermatozoa bound perivitelline membrane. Finally, regardless groups similar levels reactive oxygen species (ROS). conclusion, at tested concentrations, does bring benefits freezability, increase concentration resulted negative motility semen.