Short‐Term Storage of Collared Peccary Semen in a Transport Container at 5°C Using Different Extenders

• DOI: 10.1111/rda.70036
• Publication Date: 2025-03-12T04:41:22Z
• Authors: Romário Parente d...; Samara Sandy Jerô...; Luana Grasiele Pe...; Yuri Gonçalves Matos; Nayra Rachel Nasc...; Pedro Augusto Pin...; Pedro Nacib Jorge...; Alexandre Rodrigu...
• Keywords: Electroejaculation; Membrane integrity; Semen extender

The advancement of short-term storage methods for collared peccary semen targets its potential application in artificial insemination programmes and combination with cryopreservation techniques. objective this study was to evaluate the performance a transport container (Botutainer) preservation using commercial extenders (BTS, NUTRIXcell+ PRIMXcell Ultra) as well TRIS + egg yolk extender. Ten ejaculates obtained by electroejaculation were diluted stored at 5°C 72 h. cooled samples analysed kinetic aspects (using computerised system), membrane functionality (hyposmotic test), integrity mitochondrial activity (fluorescent probes), morphology (Rose Bengal staining) sperm binding capacity (to perivitelline yolk). After h storage, TRIS, NUTRIXcell preserved approximately 60% motile 50%-65% integrity. In contrast, BTS not effective maintaining these parameters, preserving only ~40% 50%, respectively (p < 0.05). All ~50% 65% 72%-78% normal up extender that ~75% Finally, exhibited reduction after 48 0.05), whereas other showed results comparable fresh semen. summary, we demonstrate effectiveness Botutainer device extenders.