Peptide‐induced HLA‐E expression in human PBMCs is dependent on peptide sequence and the HLA‐E genotype

• DOI: 10.1111/tan.12525
• Publication Date: 2015-03-04T11:43:47Z
• Authors: N. Lauterbach; L. Wieten; H. E. Popeijus; J. Vanderlocht; P. M. H. van Zon; C. E. M. Voorter; M. G. J. Tilanus
• Keywords: HLA-DR; HLA-G

Abstract Human Leukocyte Antigen ( HLA )‐E is a low‐polymorphic non‐classical class I molecule which plays crucial role in immune surveillance by presentation of peptides to T and natural killer NK ) cells. ‐E polymorphism related surface expression associated with patient outcome after stem cell transplantation. We aim investigate the regulation level peripheral blood mononuclear cells PBMCs healthy individuals homozygous for ‐E*01:01 or ‐E*01:03 , using panel binding derived from CMV Hsp60 I. Basal peptide‐induced levels were higher PBMC subjects as compared subjects. mRNA comparable between two genotypes remained constant peptide stimulation. seemed be not only dependent on genotype, but also sequence evidenced profound difference upregulation B7 peptide. Our results showed that regulated at posttranscriptional extracellular stimulation did influence RNA expression. This study provides new insights mechanism underlines inducing translation, may represent defense against lytic viral infections necrosis.