Evidence for Tunisian-Like Pestiviruses Presence in Small Ruminants in Italy Since 2007

• DOI: 10.1111/tbed.12498
• Publication Date: 2016-04-04T04:35:40Z
• Authors: S. Ciulli; G. Purpari; S. Agnello; P. Di Marco; S. Di Bella; E. Volpe; F. Mira; A. C. de Aguiar S...; S. Vullo; A. Guercio
• Keywords: Goat Diseases; Sheep; Genotype; Sequence Analysis, RNA; Border disease virus; Bovine viral diarrhoea virus; Classical swine fever virus; Goats; Pestivirus; Phylogenetic analysis; Sheep; Tunisian-like pestivirus; Veterinary (all); Immunology and Microbiology (all); Diarrhea Virus 1, Bovine Viral; Goats; Pestivirus Infections; Sheep Diseases; Genome, Viral; 04 agricultural and veterinary sciences; 3. Good health; 0403 veterinary science; Pestivirus; Animals; RNA, Viral; Sicily; Phylogeny

The genus Pestivirus, which belongs to the Flaviviridae family, includes ssRNA+ viruses responsible for infectious diseases in pigs, cattle, sheep, goats and other domestic wild ruminants. Like most of RNA viruses, pestivirus has high genome variability with practical consequences on disease epidemiology, diagnosis control. In addition officially recognized species such as BVDV-1, BVDV-2, BDV CSFV, pestiviruses have been detected. Furthermore, ruminant show low or absent specificity observed serological tests are able infect multiple species. Particularly, small ruminants receptive hosts heterogeneous group pestiviruses. aim this study was carry out molecular characterization isolated from sheep Sicily, Italy. Phylogenetic analysis two viral genomic regions (a fragment 5′-UTR whole Npro regions) revealed presence different genotypes analysed goat herds. Two five isolates were clustered BVDV-1d a strain widespread Italy, but never reported Sicily. three formed distinct cluster similarity Tunisian isolates, recently proposed new This represents first evidence Tunisian-like one collected goat, representing isolation