Pathogenicity of porcine deltacoronavirus (PDCoV) strain NH and immunization of pregnant sows with an inactivated PDCoV vaccine protects 5‐day‐old neonatal piglets from virulent challenge

• DOI: 10.1111/tbed.13369
• Publication Date: 2019-09-21T15:44:45Z
• Authors: Jialin Zhang; Jianfei Chen; Ye Liu; Shi Da; Hongyan Shi; Xin Zhang; Jianbo Liu; Liyan Cao; Xiangdong Zhu; Xiaobo Wang; Zhaoyang Ji; Li Feng
• Keywords: Strain (injury)

In this study, the pathogenicity of porcine deltacoronavirus (PDCoV) strain NH (passage 10, P10) was evaluated. We found that PDCoV is enteropathogenic in 5-day-old pigs. Pathogenicity experiments provided a challenge model for studying protection efficiency passive immunity. order to investigate protective efficacy immunity newborn piglets, pregnant sows were vaccinated with either PDCoV-inactivated vaccine at Houhai acupoint (n = 5) or DMEM as negative control 2) using prime/boost strategy 20 and 40 days before delivery. spike (S)-specific IgG neutralizing antibody (NA) responses detected immunized piglets born sows. sIgA also colostrum milk Five post-farrowing, orally challenged (105 TCID50/piglet). Severe diarrhoea, high levels viral RNA copies substantial intestinal villus atrophy unimmunized Only 4 31 (12.9%) group displayed mild moderate lower minor villi damage compared post-challenge. Mock exhibited no typical clinical symptoms. The experiment results indicated inactivated 87.1% piglets. These findings suggest has potential be an effective vaccine, providing against virulent PDCoV.