Frequency of human platelet antigens (HPA)‐1, ‐2, ‐5 and ‐15 in Brazilian blood donors and establishment of a panel of HPA‐typed donors

• DOI: 10.1111/tme.12210
• Publication Date: 2015-06-03T00:39:42Z
• Authors: C. M. B. Mangerona; F. B. Garcia; H. Moraes‐Souza
• Keywords: Neonatal alloimmune thrombocytopenia

SUMMARY Objectives The aim of this study is to keep a record regular human platelet antigens ( HPA )‐typed blood donors and compare their allele frequencies with those reported in other populations. Background are polymorphisms expressed on membrane glycoproteins. They can generate an immune response leading alloimmunisation that may show clinical manifestations, such as neonatal alloimmune thrombocytopenia, post‐transfusion purpura refractoriness. Platelet not uncommon, therefore, for optimum management, it advantageous establish panel typed platelets help matched selection ‐alloimmunised patients transfusion. Methods polymerase chain reaction PCR )‐allele‐specific primers ‐restriction fragment length polymorphism methods were used determine the genotypes ‐1, ‐2, ‐5 ‐15 systems 337 donors. Results three AA , AB BB ) found all analysed, most frequent ‐2 (mean: 0·732) system 0·523). Allele 0·148, 0·155, 0·140 0·430 ‐1b, ‐2b, ‐5b ‐15b, respectively, they similar Caucasian populations, especially ‐1. However, B was more when compared Amazon Indians, frequency our higher ‐1 lower comparison sub‐Saharan African Conclusions A ‐typed would enable rapid identification ‐compatible required