D variants in the population of D‐negative blood donors in the north‐eastern region of Croatia

• DOI: 10.1111/tme.12726
• Publication Date: 2020-10-09T09:34:13Z
• Authors: Hana Safic Stanic; Vesna Dogic; Ivona Herceg; Sandra Jagnjic; Jasna Bingulac‐Po...; Ivana Babic; Ante Corusic; Irena Jukic
• Keywords: Blood type (non-human)

The aim of this study was to determine RHESUS D GENE (RHD) allelic variants among Croatian D-negative blood donors and compare our results with respective data from other European countries.Altered or reduced antigen expression can result in variants, which be mistyped lead the alloimmunisation recipient. RHD genotyping distinguish variants: weak D, partial DEL, thus preventing alloimmunisation.A total 6523 samples obtained were screened for presence using real-time polymerase chain reaction (PCR) method. PCR-SSP performed variant by commercial kits (Inno-Train, Kronberg, Germany). Genomic DNA sequencing all 10 exons when failed assign a variant.RHD molecular screening revealed 23 (0.35%) RHD-PCR positive samples, C/E positive, decreasing frequency: 11 hybrid RHD-CE (2-9) D-CE 4 type 2 2. Six remained unresolved sequenced. For 12 (excluding large hybrids), an adsorption/elution anti-D serum performed, confirming that RhD+. calculated frequency clinically significant alleles RhD-negative 1:543 (0.18%) 1:53 (1.89%) donors.Data on serologically north-eastern region Croatia could help introducing routine workflow.