Apical F‐actin‐regulated exocytic targeting of NtPPME1 is essential for construction and rigidity of the pollen tube cell wall

• DOI: 10.1111/tpj.12300
• Publication Date: 2013-07-30T07:44:17Z
• Authors: Hao Wang; Xiaohong Zhuang; Yi Cai; Alice Y. Cheung; Liwen Jiang
• Keywords: Tip growth

In tip-confined growing pollen tubes, delivery of newly synthesized cell wall materials to the rapidly expanding apical surface requires spatial organization and temporal regulation F-actin filament exocytosis. this study, we demonstrate that is essential for rigidity construction tube by regulating exocytosis Nicotiana tabacum pectin methylesterase (NtPPME1). Wortmannin disrupts in tip inhibits polar targeting NtPPME1, which subsequently alters pectic composition wall, finally causing growth arrest tube. addition mechanistically linking F-actin, wortmannin can be used as a useful tool studying endomembrane trafficking cytoskeletal tubes.