Spatio‐temporal analysis of cellulose synthesis during cell plate formation in Arabidopsis

• DOI: 10.1111/tpj.12362
• Publication Date: 2013-10-22T18:44:44Z
• Authors: Fabien Miart; Thierry Desprez; Eric Biot; Halima Morin; Katia Belcram; Herman Höfte; Martine Gonneau; Samantha Vernhettes
• Keywords: Phragmoplast; Cell plate; Organelle

During cytokinesis a new crosswall is rapidly laid down. This process involves the formation at cell equator of tubulo-vesicular membrane network (TVN). TVN evolves into tubular (TN) and planar fenestrated sheet, which extends its periphery before fusing to mother wall. The role wall polymers in plate assembly poorly understood. We used specific stains GFP-labelled cellulose synthases (CESAs) show that cellulose, as well three distinct CESAs, accumulated already stage. early presence suggests extruded structures TVN. Co-localisation studies using GFP-CESAs suggest delivery synthase complexes (CSCs) via phragmoplast-associated vesicles. In more mature TN part plate, we observed GFP-CESA from doughnut-shaped organelles, presumably Golgi bodies. conversion density diminished, whereas levels remained high zone expanding plate. retrieval clathrin-containing central plasma cell, may contribute recycling CESAs peripheral growth These observations, together with mutant phenotypes cellulose-deficient mutants pharmacological experiments, key for synthesis stages assembly.